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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

B16 melanoma    Cell lines Adherent Mouse

Tissue : Mouse skin melanoma cell line.

Molecules for transfection DNA
Cell Culture Scale 6cm dish
Amount of Transfected Molecule 1μg/well
Amount of Transfection Reagent
Cell Culture Medium D-MEM
Serum FBS
Serum Content 5%
Antibiotic ペニ-スト 50 U/mL
Additive -
Medium Change (Before transfection) -
Medium Change (After transfection) -
Cell Detachment -
Cell Dissociation Reagent
Cell Viability 51~79%
Detection Method Luciferase assay
Detection Time after Transfection 48hr
Transfection Efficiency Unmeasured
Transfection Method -
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