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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

A549    Cell lines Adherent Human

Tissue : Human Caucasian lung carcinoma

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5μg/well (DNA : Reagent = 1 : 3)
Amount of Transfection Reagent 1.5μl/well (DNA : Reagent = 1 : 3)
Cell Culture Medium D-MEM (Low Glucose)[code No. 041-29775]
Serum FBS
Serum Content 10%
Antibiotic Unused
Additive Unused
Medium Change (Before transfection) Undone
Medium Change (After transfection) -
Cell Detachment Done at transfection
Cell Dissociation Reagent Trypsin-EDTA (0.25w/w% Trypsin-1mmol/L EDTA [code No. 209-16941])
Cell Viability Unmeasured
Detection Method Fluorescence microscope Flow Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 8%
Transfection Method 1-STEP method (Reverse method)
Review
Comment Medium volume:530μl(Medium 450μl+SF-DNA complex 80μl)
Transfected molecule:YFP
Cell number:150,000 cells/well (1-STEP method)

Transfection Efficiency (FCM)
ScreenFect A:0.7%
ScreenFect A plus:8%
ScreenFect A plus+SFA P-reagent:8%
Attachment files

Data(FCM)

Quick Protocol【pDNA】【24well】