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Result of Transfection

Product

A

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.

Cell Name

HuH-7    Cell lines Adherent Human

Tissue : Well differentiated human hepato cellular carcinoma cell line

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5μg/well (DNA : Reagent = 1 : 3)
Amount of Transfection Reagent 1.5μl/well (DNA : Reagent = 1 : 3)
Cell Culture Medium D-MEM (Low Glucose)[code No. 041-29775]
Serum FBS
Serum Content 10%
Antibiotic Unused
Additive Unused
Medium Change (Before transfection) Undone
Medium Change (After transfection) -
Cell Detachment Undone at transfection
Cell Dissociation Reagent Unused
Cell Viability Unmeasured
Detection Method Fluorescence microscope Flow Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 1%
Transfection Method 2-STEP method (Forward method)
Review
Comment Medium volume:530μl(Medium 450μl+SF-DNA complex 80μl)
Transfected molecule:YFP
Cell number:99,000 cells/well (2-STEP method)

Transfection Efficiency (FCM)
ScreenFect A:0.7%
ScreenFect A plus:4.4%
ScreenFect A plus+SFA P-reagent:23.4%
CompetitorA:7.3%
CompetitorB+Additive:8%
CompetitorC:7.5%
Attachment files

[DNA_A]Quick Protocol_Eng 160906.pdf