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Result of Transfection

Product

A

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.

Cell Name

MCF-7    Cell lines Suspension Human

Tissue : Cell line derived from human breast adenocarcinoma.

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5μg/well (DNA : Reagent = 1 : 3)
Amount of Transfection Reagent 1.5μl/well (DNA : Reagent = 1 : 3)
Cell Culture Medium D-MEM (Low Glucose)[code No. 041-29775]
Serum FBS
Serum Content 10%
Antibiotic Unused
Additive Unused
Medium Change (Before transfection) Undone
Medium Change (After transfection) -
Cell Detachment Undone at transfection
Cell Dissociation Reagent Unused
Cell Viability Unmeasured
Detection Method Fluorescence microscope Flow Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 16%
Transfection Method 2-STEP method (Forward method)
Review Application Data【24 well】
Comment .Medium volume:530μl(Medium 450μl+SF-DNA complex 80μl)
Transfected molecule:YFP
Cell number:200,000 cells/well (2-STEP method)

Transfection Efficiency (FCM)
ScreenFect A:16%
ScreenFect A plus:22%
ScreenFect A plus+SFA P-reagent:33%
Attachment files

[DNA_A]Quick Protocol_Eng 160906.pdf