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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

HCT116    Cell lines Adherent Human

Tissue : Human cell line derived from colon cancer.

Molecules for transfection Plasmid DNA
Cell Culture Scale 96 wells
Amount of Transfected Molecule 0.075 μg/well (DNA : Reagent = 1 : 2)
Amount of Transfection Reagent 0,15 µl/well (DNA : Reagent = 1 : 2)
Cell Culture Medium -
Serum -
Serum Content -
Antibiotic -
Additive -
Medium Change (Before transfection) -
Medium Change (After transfection) -
Cell Detachment Done at transfection
Cell Dissociation Reagent -
Cell Viability 63%
Detection Method Fluorescence microscope
Detection Time after Transfection -
Transfection Efficiency 70%
Transfection Method 1-STEP method (Reverse method)
Review User feedback
Comment Medium volume:100μl(Medium 80μl+SF-DNA complex 20μl)
Transfected molecule:GFP
Cell number:30,000 cells/well (1-STEP method)