Back

Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

A549    Cell lines Adherent Human

Tissue : Human Caucasian lung carcinoma

Molecules for transfection Plasmid DNA
Cell Culture Scale 96 wells
Amount of Transfected Molecule 0.075μg/well(DNA : Reagent = 1 : 3)
Amount of Transfection Reagent 0.225μl/well(DNA : Reagent = 1 : 3 )
Cell Culture Medium RPMI1640 [code No. 189-02025]
Serum FCS
Serum Content 10%
Antibiotic Unused
Additive Unused
Medium Change (Before transfection) Done
Medium Change (After transfection) -
Cell Detachment Done at transfection
Cell Dissociation Reagent Accutase®
Cell Viability 70%
Detection Method GFP Flow Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 57%
Transfection Method 1-STEP method (Reverse method)
Review User feedback
Comment Medium Volume:100μl(Medium80μl+SF-DNA complex 20μl)
Transfected molecule:GFP
Cell number:35,000 cells/well (1-STEP method)