Back

Result of Transfection

Product

A

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.

Cell Name

Hep G2    Cell lines Adherent Human

Tissue : Human hepatoma cell line

Notes : http://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=85011430&collection=ecacc_gc

Molecules for transfection Plasmid DNA
Cell Culture Scale 96 wells
Amount of Transfected Molecule 0.075μg/well(DNA : Reagent = 1 : 4)
Amount of Transfection Reagent 0.3μl/well(DNA : Reagent = 1 : 4 )
Cell Culture Medium MEM Alpha
Serum FCS
Serum Content 10%
Antibiotic Unused
Additive Unused
Medium Change (Before transfection) Done
Medium Change (After transfection) -
Cell Detachment Done at transfection
Cell Dissociation Reagent Accutase®
Cell Viability 73%
Detection Method GFP Flow Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 41%
Transfection Method 1-STEP method (Reverse method)
Review User feedback
Comment Medium Volume:100μl(Medium80μl+SF-DNA complex 20μl)
Transfected molecule:GFP
Cell number:30,000~35,000 cells/well (1-STEP method)