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Result of Transfection

Product

A

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.

Cell Name

4T1    Cell lines Adherent Mouse

Tissue : Mammary gland

Notes : https://www.atcc.org/Products/All/CRL-2539.aspx

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.3μg/well(DNA : Reagent = 1:3 )
Amount of Transfection Reagent 1μl/well(DNA : Reagent = 1:3 )
Cell Culture Medium RPMI1640
Serum FBS
Serum Content 10%
Antibiotic uesd
Additive Unused
Medium Change (Before transfection) Done
Medium Change (After transfection) Undone
Cell Detachment Done at transfection
Cell Dissociation Reagent TrypLE Express
Cell Viability 97%
Detection Method GFP Imaging Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 26%
Transfection Method 1-STEP method (Reverse method)
Review User feedback
Comment Medium Volume:530μl(Medium450μl+SF-DNA complex 80μl)
Transfected molecule:GFP
Cell number:100,000 cells/well (1-STEP method)