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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

4T1    Cell lines Adherent Mouse

Tissue : Mammary gland

Notes : https://www.atcc.org/Products/All/CRL-2539.aspx

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5μg/well(DNA : Reagent = 1:4 )
Amount of Transfection Reagent 2μl/well(DNA : Reagent = 1:4 )
Cell Culture Medium RPMI1640
Serum FBS
Serum Content 10%
Antibiotic uesd
Additive Unused
Medium Change (Before transfection) Done
Medium Change (After transfection) Undone
Cell Detachment Done at transfection
Cell Dissociation Reagent TrypLE Express
Cell Viability 96%
Detection Method GFP Imaging Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 30%
Transfection Method 1-STEP method (Reverse method)
Review User feedback
Comment Medium Volume:530μl(Medium450μl+SF-DNA complex 80μl)
Transfected molecule:GFP
Cell number:100,000 cells/well (1-STEP method)