Back

Result of Transfection

Product

A

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.

Cell Name

A172    Cell lines Adherent Human

Tissue : Human glioblastoma, nervous

Notes : https://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=88062428&collection=ecacc_gc

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5 μg/well(DNA : Reagent = 1:4 )
Amount of Transfection Reagent 2.0 μl/well (DNA : Reagent = 1 : 4)
Cell Culture Medium D-MEM
Serum FBS
Serum Content 8%
Antibiotic Unused
Additive 2 mmol/l L-Ala-L-Glu
Medium Change (Before transfection) Undone
Medium Change (After transfection) Undone
Cell Detachment Undone at transfection
Cell Dissociation Reagent Trypsin-EDTA
Cell Viability 80~90%
Detection Method GFP
Detection Time after Transfection 48hr
Transfection Efficiency 20% - 10%
Transfection Method 2-STEP method (Forward method)
Review User feedback
Comment Good Transfection Efficiency and Good Cost Performance
Attachment files

Quick Protocol【pDNA】