Back

Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

A172    Cell lines Adherent Human

Tissue : Human glioblastoma, nervous

Notes : https://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=88062428&collection=ecacc_gc

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5 μg/well(DNA : Reagent = 1:4 )
Amount of Transfection Reagent 2.0 μl/well (DNA : Reagent = 1 : 4)
Cell Culture Medium D-MEM
Serum FBS
Serum Content 8%
Antibiotic Unused
Additive 2 mmol/l L-Ala-L-Glu
Medium Change (Before transfection) Undone
Medium Change (After transfection) Undone
Cell Detachment Undone at transfection
Cell Dissociation Reagent Trypsin-EDTA
Cell Viability 80~90%
Detection Method GFP
Detection Time after Transfection 48hr
Transfection Efficiency 20% - 10%
Transfection Method 2-STEP method (Forward method)
Review User feedback
Comment Good Transfection Efficiency and Good Cost Performance
Attachment files

Quick Protocol【pDNA】