Back

Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

3T3-L1    Cell lines Adherent Mouse

Tissue : Derived from 3T3-Swiss mouse fibroblast

Molecules for transfection DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5 μg/well
Amount of Transfection Reagent -
Cell Culture Medium D-MEM
Serum FBS
Serum Content 10%
Antibiotic 0.5% PenicillinG-Streptomycin
Additive -
Medium Change (Before transfection) Undone
Medium Change (After transfection) -
Cell Detachment Undone at transfection
Cell Dissociation Reagent -
Cell Viability 80~100%
Detection Method Fluorescence microscope
Detection Time after Transfection 72hr
Transfection Efficiency 10% - 1%
Transfection Method 2-STEP method (Forward method)
Review User Feedback
Comment 72 hours with a medium change after 48 hours