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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

Flounder hematopoietic cells    Cell lines Suspension Flounder

Tissue : Flounder

Molecules for transfection DNA
Cell Culture Scale 12 wells
Amount of Transfected Molecule 500 ng/well
Amount of Transfection Reagent -
Cell Culture Medium eRDF
Serum FBS
Serum Content 20%
Antibiotic Used
Additive ヒラメ血清
Medium Change (Before transfection) -
Medium Change (After transfection) -
Cell Detachment -
Cell Dissociation Reagent -
Cell Viability Unmeasured
Detection Method Fluorescence microscope
Detection Time after Transfection Over 48hr
Transfection Efficiency -
Transfection Method
Review User feedback
Comment I couldn't get good results in this case.