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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

CHO-K1    Cell lines Adherent Hamster

Tissue : Subspecific cell line of CHO (Chinese Hamster Ovary Cell line).

Molecules for transfection Plasmid DNA
Cell Culture Scale -
Amount of Transfected Molecule -
Amount of Transfection Reagent -
Cell Culture Medium -
Serum -
Serum Content -
Antibiotic -
Additive -
Medium Change (Before transfection) -
Medium Change (After transfection) -
Cell Detachment -
Cell Dissociation Reagent -
Cell Viability -
Detection Method Fluorescence microscope Flow Cytometer Western blotting
Detection Time after Transfection
Transfection Efficiency 20% - 10%
Transfection Method 2-STEP method (Forward method)
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