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Result of Transfection

Product

A

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.

Cell Name

CHO-K1    Cell lines Adherent Hamster

Tissue : Subspecific cell line of CHO (Chinese Hamster Ovary Cell line).

Molecules for transfection Plasmid DNA
Cell Culture Scale 24 wells
Amount of Transfected Molecule 0.5 μg/well (DNA : Reagent = 1 : 3)
Amount of Transfection Reagent 1.5 μl/well (DNA : Reagent = 1 : 3)
Cell Culture Medium Ham's F-12K with L-Glutamine, Phenol Red and Sodium Pyruvate [code No. 080-08565]
Serum FBS
Serum Content 10%
Antibiotic Unused
Additive Unused
Medium Change (Before transfection) Undone
Medium Change (After transfection) -
Cell Detachment Undone at transfection
Cell Dissociation Reagent Unused
Cell Viability Unmeasured
Detection Method Fluorescence microscope Flow Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 38% - 34%
Transfection Method 2-STEP method (Forward method)
Review Good Cost Performance
Comment Medium volume:530μl(Medium 450μl+SF-DNA complex 80μl)
Transfected molecule:YFP
Cell number:50,000 cells/well (2-STEP method)

Transfection Efficiency (FCM)
ScreenFect A:36%
ScreenFect A+SFA P-reagent:25%
ScreenFect A plus:54%
ScreenFect A plus+SFA P-reagent:38%
CompetitorA:55%
CompetitorB+Additive:46%
CompetitorC:53%
Attachment files

CHO-K1.2-step.FCM.Fluorescence(-).pdf

[DNA_A]Quick Protocol_Eng 160906.pdf