Result of Transfection

Product	A plus DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.
Cell Name	A549 Cell lines Adherent Human Tissue : Human Caucasian lung carcinoma
Molecules for transfection	Plasmid DNA
Cell Culture Scale	24 wells
Amount of Transfected Molecule	0.5μg/well (DNA : Reagent = 1 : 3)
Amount of Transfection Reagent	1.5μl/well (DNA : Reagent = 1 : 3)
Cell Culture Medium	D-MEM (Low Glucose)［code No. 041-29775］
Serum	FBS
Serum Content	10%
Antibiotic	Unused
Additive	Unused
Medium Change (Before transfection)	Undone
Medium Change (After transfection)	-
Cell Detachment	Done at transfection
Cell Dissociation Reagent	Trypsin-EDTA （0.25w/w% Trypsin-1mmol/L EDTA [code No. 209-16941]）
Cell Viability	Unmeasured
Detection Method	Fluorescence microscope Flow Cytometer
Detection Time after Transfection	24hr
Transfection Efficiency	8%
Transfection Method	1-STEP method (Reverse method)
Review
Comment	Medium volume：530μl（Medium 450μl+SF-DNA complex 80μl） Transfected molecule：YFP Cell number：150,000 cells/well (1-STEP method) Transfection Efficiency (FCM) ScreenFect A：0.7% ScreenFect A plus：8% ScreenFect A plus+SFA P-reagent：8%
Attachment files	【DNA】Quick Protocol_Eng 160805.pdf