Result of Transfection



DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.

Cell Name

Caco-2    Cell lines Adherent Human

Tissue : colon (Human Caucasian colon adenocarcinoma)

Molecules for transfection Plasmid DNA
Cell Culture Scale 96 wells
Amount of Transfected Molecule 0.075 μg/well (DNA : Reagent = 1 : 4)
Amount of Transfection Reagent 0.3 μl/well (DNA : Reagent = 1 : 4)
Cell Culture Medium -
Serum -
Serum Content -
Antibiotic -
Additive -
Medium Change (Before transfection) -
Medium Change (After transfection) -
Cell Detachment Done at transfection
Cell Dissociation Reagent -
Cell Viability 91%
Detection Method Fluorescence microscope
Detection Time after Transfection -
Transfection Efficiency 41%
Transfection Method 1-STEP method (Reverse method)
Review User feedback
Comment Medium volume:100μl(Medium 80μl+SF-DNA complex 20μl)
Transfected molecule:GFP
Cell number:20,000 cells/well (1-STEP method)