DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.
C2C12 Cell lines Adherent Mouse
Tissue : Mouse myoblast cell line
|Molecules for transfection||Plasmid DNA|
|Cell Culture Scale||48 wells|
|Amount of Transfected Molecule||0.3 μg/well (DNA : Reagent = 1：3)|
|Amount of Transfection Reagent||0.9 μl/well (DNA : Reagent = 1 : 3)|
|Cell Culture Medium||D-MEM|
|Antibiotic||PenicillinG (100 units/ml)-Streptomycin (100 μg/ml)|
|Medium Change (Before transfection)||Undone|
|Medium Change (After transfection)||Done|
|Cell Detachment||Done at transfection|
|Cell Dissociation Reagent||Trypsin-EDTA|
|Detection Method||Western blotting|
|Detection Time after Transfection||48hr|
|Transfection Efficiency||80% - 70%|
|Transfection Method||1-STEP method (Reverse method)|
Good Transfection Efficiency and Good Cost Performance.
12 hours after transfection, the medium was replaced with serum-containing medium.