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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

C2C12    Cell lines Adherent Mouse

Tissue : Mouse myoblast cell line

Molecules for transfection Plasmid DNA
Cell Culture Scale 48 wells
Amount of Transfected Molecule 0.3 μg/well (DNA : Reagent = 1:3)
Amount of Transfection Reagent 0.9 μl/well (DNA : Reagent = 1 : 3)
Cell Culture Medium D-MEM
Serum FBS
Serum Content 10%
Antibiotic PenicillinG (100 units/ml)-Streptomycin (100 μg/ml)
Additive -
Medium Change (Before transfection) Undone
Medium Change (After transfection) Done
Cell Detachment Done at transfection
Cell Dissociation Reagent Trypsin-EDTA
Cell Viability 80~90%
Detection Method Western blotting
Detection Time after Transfection 48hr
Transfection Efficiency 80% - 70%
Transfection Method 1-STEP method (Reverse method)
Review User feedback
Comment Good Transfection Efficiency and Good Cost Performance.
12 hours after transfection, the medium was replaced with serum-containing medium.
Attachment files

【DNA】Quick Protocol_Eng 160805.pdf