Result of Transfection

Product	A plus DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.
Cell Name	C2C12 Cell lines Adherent Mouse Tissue : Mouse myoblast cell line
Molecules for transfection	Plasmid DNA
Cell Culture Scale	48 wells
Amount of Transfected Molecule	0.3 μg/well (DNA : Reagent = 1：3)
Amount of Transfection Reagent	0.9 μl/well (DNA : Reagent = 1 : 3)
Cell Culture Medium	D-MEM
Serum	FBS
Serum Content	10%
Antibiotic	PenicillinG (100 units/ml)-Streptomycin (100 μg/ml)
Additive	-
Medium Change (Before transfection)	Undone
Medium Change (After transfection)	Done
Cell Detachment	Done at transfection
Cell Dissociation Reagent	Trypsin-EDTA
Cell Viability	80～90%
Detection Method	Western blotting
Detection Time after Transfection	48hr
Transfection Efficiency	80% - 70%
Transfection Method	1-STEP method (Reverse method)
Review	User feedback
Comment	Good Transfection Efficiency and Good Cost Performance. 12 hours after transfection, the medium was replaced with serum-containing medium.
Attachment files	【DNA】Quick Protocol_Eng 160805.pdf