Result of Transfection

Product	A DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells.
Cell Name	4T1 Cell lines Adherent Mouse Tissue : Mammary gland Notes : https://www.atcc.org/Products/All/CRL-2539.aspx
Molecules for transfection	Plasmid DNA
Cell Culture Scale	24 wells
Amount of Transfected Molecule	0.3 μg/ well (DNA : Reagent = 1 : 3 )
Amount of Transfection Reagent	1 μl/ well (DNA : Reagent = 1 : 3)
Cell Culture Medium	RPMI1640
Serum	FBS
Serum Content	10%
Antibiotic	Used
Additive	-
Medium Change (Before transfection)	Done
Medium Change (After transfection)	Undone
Cell Detachment	Done at transfection
Cell Dissociation Reagent	TrypLE Express
Cell Viability	97%
Detection Method	GFP Imaging Cytometer
Detection Time after Transfection	24hr
Transfection Efficiency	26%
Transfection Method	1-STEP method (Reverse method)
Review
Comment	Transfected molecule：GFP Cell number：100,000cells/well (1-STEP method)