DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.
CHO-K1 Cell lines Adherent Hamster
Tissue : Subspecific cell line of CHO (Chinese Hamster Ovary Cell line).
|Molecules for transfection||DNA|
|Cell Culture Scale||-|
|Amount of Transfected Molecule||-|
|Amount of Transfection Reagent||-|
|Cell Culture Medium||-|
|Medium Change (Before transfection)||-|
|Medium Change (After transfection)||-|
|Cell Dissociation Reagent||-|
|Detection Method||Fluorescence microscope|
|Detection Time after Transfection||-|
|Transfection Method||1-STEP method (Reverse method)|