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Result of Transfection

Product

A plus

DNA and siRNA can be transfected into general experimental cell strains, stem cells, blood cells, microglia, primary (initial subculture) cells, and insect cells. The optimization range (DNA : reagent ratio) is more flexible than ScreenFect™A, and transfection efficiency is also improved.

Cell Name

C32TG    Cell lines Adherent Human

Tissue : Human Amelanotic Melanoma

Notes : https://www.phe-culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=96020925&collection=ecacc_gc

Molecules for transfection Plasmid DNA
Cell Culture Scale 6 wells
Amount of Transfected Molecule 2.5 μg/ well (DNA : Reagent = 1 : 4 )
Amount of Transfection Reagent 10 μl/ well (DNA : Reagent = 1 : 4 )
Cell Culture Medium -
Serum -
Serum Content
Antibiotic
Additive -
Medium Change (Before transfection) Done
Medium Change (After transfection) -
Cell Detachment Done at transfection
Cell Dissociation Reagent -
Cell Viability 12%
Detection Method GFP Flow Cytometer
Detection Time after Transfection 24hr
Transfection Efficiency 42%
Transfection Method 1-STEP method (Reverse method)
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